如何用Origin拟合标准曲线并计算IC50？ 详细 0 3
推荐回答（1个）
但实际上在计算IC50 的同时，我们一般也要标准曲线，还要线性方程。所以还是用Origin 来拟合标准曲
线，然后用origin 内置功能来计算IC50 比较方便。
这里使用Origin7.5 来作演示。
1. 先随意输入一组数据吧
2. 选中这2 列数据，然后点左下角的作scatter 图的图标，
3. 然后就生成了散点图
4. 点击Analysis 菜单中的Fit Polynomial，在弹出的对话框中，Order 处设为1，这样就是作线性拟合，
就是勾选对话框中的Show Flormula on graph。（可能Origin 的设计者认为线性拟合公式太简单，默
认就不用显示了）
5. 点击OK 后，就得到了拟合后的图形。线性方程公式也显示在了图形上。注意窗口的右下角。
点击那里的小箭头后，我们可以看到完整的拟合统计信息，如相关系数R2=0.9918
6. 好了，标准曲线知道了，现在就来计算IC50。根据IC50 定义，该例子中就是Y 取中值时，X 对应的
数值，这里Y 的中值是0.6，那根据线性方程就可以自己算出来对应的X 值。那如果不是线性方程，公式
比较复杂手工计算就很麻烦了，所以还是用Origin 中的功能吧。
点击Tools，Linear Fit， 如果不是线性拟合的，请选择其他拟合方式，如果是S 形曲线的，则需要选择
Sigmoidal Fit.
7. 在弹出的对话框中，先点击Fit，然后在Find Y 处输入0.6，点击Find X 按钮，得到的数值就是IC50
了。
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