假设ROS是解释精子在孵育过程中产生碎片的原因,Muratori发现:实验室环境下不加任何的干预措施,精子碎片化并不由内源性ROS产生运灶,我们数据显示出我们的结论与他的结论相同。我们数据显示“梯度法”6-8小时DNA碎片化速度慢于“上游法”。
Assuming that ROS is the reason for causing sperm fragmentation during the course of incubation, Muratori had discovered that under the laboratory environment without any intervention, the DNA fragmentation of spermatozoa was not caused by endogenous ROS; the conclusion presented by our data coincides with his conclusion. Our data shows that by applying 6-8 hours of “gradient centrifugation” method, the speed of DNA fragmentation is slower than that by the “swim up” method.
当我们对比这两种方法时,这两含和条线的角度大小说明了他们碎片化速度。这两种方法都可以筛选出活动力好的旁老扮精子,去除没有活力的精子及坏死精子,但是只有梯度法才能去除不成熟精子。另一方面,电镜显示不成熟精子存在残余细胞质,这是ROS产生的重要原因。
When we compare these two methods, the angle sizes of these two lines explain the speed of their DNA fragmentations. Both of these two methods can be used to select spermatozoa of good motility and to remove the inactive and necrotic spermatozoa; but the immature spermatozoa can only be removed by the “swim up” method. On the other hand, electron microscopic observation has shown that there are persistent cytoplasmic residues in immature spermatozoa; this is the important factor for the generation of ROS.
【英语牛人团】
建议你可以用GOOGLE的翻译。还挺不错的。
Hypothesis is explained in ROS sperm incubation process, the reasons of fragments Muratori found: laboratory environment without any intervention measures, sperm fragmentation is not produced by endogenous ROS, our data shows that we conclusion and his conclusion the same. Our data shows "gradient method" 6 to 8 hours DNA fragmentation speeds slower than "upstream method". When we compare the two kinds of method, the two lines from the point of view of the size indicates their fragmentation speed. The two methods can be decreased activity were good sperm, remove the sperm and necrosis no vitality, but only sperm gradient method to remove not mature sperm. On the other hand, electron microscopy showed not mature sperm exist, this is the residual cytoplasm ROS produced important reasons