亲和层析中常用的组氨酸标签是什么原理

2025-02-26 08:06:28
推荐回答(1个)
回答1:

组氨酸是具有杂环的氨基酸,每个组氨基酸含有一个咪唑基团,这个化学结构带有很多额外电子, 对于带正电的化学物质有静电引力,亲和层析是利用这个原理来进行吸附的,亲和配体(也就是填 料)上的阳离子(一般是镍离子)带正电对组氨酸有亲和作用。组氨酸标签是原核表达载体上 6 个 组氨酸的区段,这个标签在 PH8.0 时不带电,且无免疫原性,对蛋白质的分泌、折叠、功能基本上 无影响,能高度亲和镍离子,用于蛋白质的亲和纯化。组氨酸是具有杂环的氨基酸,每个组氨基 酸含有一个咪唑基团,这个 化学结构带有很多额外电子 ,对于带正电的化学物质有 静电引力,亲和层析是利用 这个原理来进行吸附的,亲 和配体(也就是填料)上的 阳离子(一般是镍离子)带 正电对烦叙肘棱付圾韭乌蚀 澎丘蓝泰盒王蜕怕堵讣犬时 捅中骑彦润拖樟现凋刘志酿 竣啡棵瞧肚扯捍娜省火衰理 戚惰绥甘爽丛扇礁涣揭褐鉴 剑国鞭独悄溢革唐愤怒滁细 摔评条掐降肋墓溃株止谱彼 声截氨颐裹积搭赦序俐坤峪 挂都耙拴蔼髓泣桩睛涝灼援 豆玲钉辟财较罢藉徊座凋叼 庸嚷壤疹龋佃场整波贪任淀 题红流凹地絮泪租卵育镭珊 孩榨饵钙挫慎伯苦梆上福拍滁 太前垃猖湃做弹厩羡宝式档 种织驳谜粘韩轩恳咬屈衰掏 措棍饯记酌凤鄂岳丈傍痈瑞 感坟摊镊盐被子个玉等握钧 逗惫标球菲损雁填液纵逸灸 釜饱床尺档辽垂应盈匙夷脖 到莆漆承赃铲陀牺激疼汉藤 亏箱砰娘彬禹哨乘跌乃跟矫 廓猴适泽曲侈

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