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LB培养基的分类

2025-03-15 01:38:13

推荐回答（1个）

回答1：

根据《分子克隆实验指南》（J.萨姆布鲁克D.W.拉塞尔著）配制每升培养基,应该在950 ml去离子水中加入:
胰蛋白胨10g
酵母提取物5g
NaCl 10g
摇动容器直至溶质溶解.用5mol/LNaOH调pH至7.0.用去离子水定容至1L.在15psi高压下蒸汽灭菌21min.
培养的菌种一般是经过改造的无法在外界环境单独存活和扩增的工程菌.通过培养工程菌,我们可以表达大量的外源蛋白,也可以拿到带有外源基因的质粒,工程菌的有效扩增是生化分子实验的基础.
LB培养基的配方如下:
胰蛋白胨(Tryptone) 10g/L
酵母提取物(Yeast extract) 5g/L
氯化钠(NaCl) 10g/L
另外根据经验值用NaOH调节该培养基的pH,使其达到7.4(该pH适合目前使用最广的原核表达菌种E.coli的生长) LB固体培养基1L和液体一样，加10g~15g琼脂粉，一定要在温度降下之前加好抗生素，并且倒好板。

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